روش تعیین مقدار نوکلئوتیدهای حلقوی cgmp , camp

(تغییرات نوکلئوتیدهای حلقوی در دیابتی های ایران)

Identification of novel target proteins of cGMP/cAMP signaling pathway using cGMP/cAMP capture compound mass spectrometry (CCMS)

Background The cyclic nucleotide monophosphates cGMP and cAMP play an essential role in many signaling pathways. The identification and profiling of cGMPand cAMP-binding proteins is an important step in order to elucidate the molecular basis of these pathways. In order to develop a tool to specifically target cGMPand cAMP-binding proteins, we synthesized cGMPand cAMP Capture CompoundsTM (CCs). ...

PDE5 modulates oocyte spontaneous maturation via cGMP-cAMP but not cGMP-PKG signaling.

Phosphodiesterase type 5 (PDE5), a cGMP specific, cGMP binding phosphodiesterase, specifically hydrolyzes cGMP to 5'-GMP. Here, we examine the distribution of PDE5 in mouse ovary and its effects on spontaneous maturation of mouse oocytes. PDE5 is present in oocytes and cumulus cells of big, antral follicles. Inhibition of activity of PDE5 significantly and reversibly inhibits spontaneous matura...

Differential regulation of Paramecium ciliary motility by cAMP and cGMP

cAMP and cGMP had distinct effects on the regulation of ciliary motility in Paramecium. Using detergent-permeabilized cells reactivated to swim with MgATP, we observed effects of cyclic nucleotides and interactions with Ca2+ on the swimming speed and direction of reactivated cells. Both cAMP and cGMP increased forward swimming speed two- to threefold with similar half-maximal concentrations nea...

cAMP modulates cGMP-mediated cerebral arteriolar relaxation in vivo.

No studies have specifically addressed whether cAMP can influence nitric oxide (NO)/cGMP-induced cerebral vasodilation. In this study, we examined whether cAMP can enhance or reduce NO-induced cerebral vasodilation in vivo via interfering with cGMP efflux or through potentiating phosphodiesterase 5 (PDE5)-mediated cGMP breakdown, respectively, in cerebral vascular smooth muscle cells (CVSMCs). ...

IL-4 induces cAMP and cGMP in human monocytic cells

Human monocytes, preincubated with IFN-gamma respond to IL-4 by a cGMP increase through activation of an inducible NO synthase. Here, IL-4 was found to induce an accumulation of cGMP (1 - 3 min) and cAMP (20 - 25 min) in unstimulated monocytes. This was impaired with NOS inhibitors, but also with EGTA and calcium/calmodulin inhibitors. These results suggest that: (1) IL-4 may stimulate differen...